A variety of microorganisms can be found in swimming pools, which may be introduced in the pool water in a number of ways. The microbial analysis of three different swimming pool in Ago-Iwoye, Ijebu-North Local Government Area of Ogun State Nigeria were studied using standard procedure techniques. Microorganisms recovered from the samples are Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, Shigella species, Streptococcus feacalis, Bacillus species, Enterococcus species and Vibro cholerae. The total coliform count of the water samples ranged from 0.88 × 103 Cfu/ml to 2.89 × 103Cfu/ml. The total viable count of the swimming pool water samples ranged from 0.50 × 103 CFU/ml to 2.40 × 103CFU/ml. These microorganism recovered are not friendly with human environment and it is therefore recommended that swimming pool water should properly treated in order to reduce microbial loads that can be threat to human health.
TABLE OF CONTENTS
CHAPTER ONE: INTRODUCTION
1.1 Background to the Study
1.2 Aim and Objective of the Study
1.3 Justification
1.4 Significance of the Study
CHAPTER TWO: LITERATURE REVIEW
2.1 Infectious diseases from Water Exposure
2.2 Commonly Used Microbial Indicators
2.2.1. Total Coliforms Bacteria
2.2.2 Fecal Coliforms Bacteria
2.2.3 Fecal Streptococci and Enterococci (FS)
2.2.4 Staphylococcus aureus
2.3 Chemical Hazards of Swimming Pool Water
2.3.1 Chlorinebased Disinfectants
2.3.2 Hydrogen ion Concentration
2.4 Current Status of Swimming Pool Waters
2.5 Evidence of Microbiological Contamination to Swimming Pool
CHAPTER THREE: MATERIALS AND METHODS
3.1 Collection of Samples
3.2 Material and Apparatus Used
3.3 Sterilization of Glasswares
3.4 Media Used
3.5 Media Preparation
3.6 Determination of Microbial Load
3.7 Identification of the Isolates
3.8 Gram Staining Method
3.9 Catalase Test
3.10 Coagulase Test
3.11 Oxidase Test
3.12 Indole Test (Kovac;s Reagent)
3.13 Urease Test
3.14 Citrate Utilization test (Citrate Test)
3.15 Methyl Red Test
3.16 Voges Proskauer Test
3.17 Starch Hydrolysis
3.18 Triple Sugar Iron (Sugar Fermentation)
CHAPTER FOUR: RESULTS
4.1 Isolates Recovered from the Samples
4.2 Frequency and Percentage of Bacteria Recovered from Swimming Pool Water Sample
4.3 Morphological and Biochemical Characteristics of Isolates Recovered from Swimming Pool Water Sample
4.4 Total Coliform Count
4.5 Total viable count
CHAPTER FIVE: DISCUSSION AND CONCLUSION
5.1 Discussion
5.2 Conclusion
References
LIST OF TABLES
4.1 Percentage Occurrence of Bacteria Recovered from Swimming Pool Water Sample
4.2 Morphological and Biochemical Reaction of Bacterial Organisms Recovered from Swimming Pool Water Sample
4.3 Total coliform count of well water samples
4.4 Total Viable Count of Swimming Pool Water Samples
