GRAM NEGATIVE BACTERIA ISOLATED FROM THE ORAL CAVITY OF DOMESTIC DOGS (Canis lupus) IN OYO STATE

GRAM NEGATIVE BACTERIA ISOLATED FROM THE ORAL CAVITY OF DOMESTIC DOGS (Canis lupus) IN OYO STATE

Project Details

GRAM NEGATIVE BACTERIA ISOLATED FROM THE ORAL CAVITY OF DOMESTIC DOGS (Canis lupus) IN OYO STATE



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Description

ABSTRACT

The aim of this study was to isolate gram negative bacteria from oral cavity of domestic dogs (Canis lupus) in Oyo state. Oral swabs from 15 dogs were inoculated on Nutrient Agar and MacConkey Agar followed by standard biochemical tests for species identification of the isolates. A total number of twenty five (25) bacteria were isolated and twelve (12) were gram negative bacteria. The most frequency occurred bacteria isolates was Klebsiella pneumonae (n=4) 33.3% and Escherichia coli (n=4) 33.3%, followed by Enterobacter spp. (n=2) 16.7%, Proteus sp. (n=1) 8.3% and Shigella sp. (n=1) 8.3%. The susceptibility of the gram negative bacteria were tested against common antibiotics. Some of the bacteria were susceptible to the antibiotic (Proteus sp. Enterobacter sp. and Shigella sp.) while some were resistant to the antibiotic (Klebseillia pneumonae and Escherichia coli).

 

 

TABLE OF CONTENTS

CHAPTER ONE: Introduction

1.1       Background to the Study

1.2       Statement of the Problem

1.3       Significance of the Study

1.4       Aim and Objective of the Study

CHAPTER TWO: Literature Review

2.1       Dog Bite Injury

2.2       Incidence of Microorganisms in Oral Cavity of Dogs

2.3       Dog Bites Incidence and Associated Risk Factors

2.3.1    Rabies

2.4       Antimicrobial Resistance of Bacteria Isolated from Oral Cavities of Dogs

2.5       Dog Bite Prevention

CHAPTER THREE: Materials and Methods

3.1       Study Area

3.2       Sample collection

3.3       Materials

3.4       Sterilization of Glassware

3.5       Media Preparation

3.6       Isolation of Bacteria

3.7       Morphological and Biochemical Characterization

3.7.1    Gram Staining

3.7.2    Citrate Utilisation Test

3.7.3    Indole Test

3.7.4    Urease Test

3.7.5    Oxidase Test

CHAPTER FOUR: Results

CHAPTER FIVE: Discussion, Results and Recommendations

5.1       Discussion

5.2       Conclusion

5.3       Recommendations

REFERENCES

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