MICROBIAL AIR-SPORA OF MICROBIOLOGY LABORATORY COMPARED WITH THE CHEMISTRY LABORATORY ON CAMPUS

MICROBIAL AIR-SPORA OF MICROBIOLOGY LABORATORY COMPARED WITH THE CHEMISTRY LABORATORY ON CAMPUS

Project Details

MICROBIAL AIR-SPORA OF MICROBIOLOGY LABORATORY COMPARED WITH THE CHEMISTRY LABORATORY ON CAMPUS



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Description

The airborne microbial concentrations of two laboratories located in the main campus of Olabisi Onabanjo University (OOU) were investigated within a period of two weeks. The total aerobic plate counts and total coliform count were determined by the spread plate method on Nutrient and Potato Dextrose Agar respectively. The Total Coliform count obtained from the samples ranged from 2.0 x 106 to 2.8 x 106 cfu/ml for Chemistry laboratory and 1.0 x 104 to 3.2 x 104 cfu/ml for Microbiology laboratory. Also total aerobic count obtained from the samples ranged from 4.3 x 104 to 9.0 x 106 cfu/ml for Chemistry laboratory and 3.2 x 106 to 5.6 x 106 cfu/ml for Microbiology laboratory. The microorganisms isolated were identified microscopically and by biochemical tests. The bacteria includes Bacillus spp., Straphylococcus spp., Klebsiella spp., Micrococcus leteus and Streptococcus spp. The fungal includes Epidermophyton spp., aspergillus niger and Penicillium spp. The study revealed that there is microbial load in both laboratories that were study. This comparative information could be useful for the school authority, laboratory instructors and public health practitioners on common airborne bacteria and fungi, and their roles in the indoor air quality of laboratories.
TABLE OF CONTENTS
CHAPTER ONE: Introduction
1.1 Background to the Study
1.2 Objective of the Study
1.3 Justification of the Study
CHAPTER TWO: Literature Review
2.1 Microbes in Indoor Environments
2.2 Moisture Damage and Microbes
2.2.1 Microbial Growth on Building Materials
2.2.3 Effects of Remediation on Microbial Indoor Environment Quality
CHAPTER THREE: Materials and Methods
3.0 Study Area
3.1 Sample Collection
3.3 Materials and Media Used
3.3.1 Instrument and glass wares
3.3.2 Media Used
3.3.3 Method of Sterilization
3.4 Culture Method
3.4.1 Isolation of Bacterial and Fungal
3.5 Biochemical method of Identification of Bacteria
3.5.1 Characterization of Bacterial Isolate
CHAPTER FOUR: Results
4.1 Total Coliform Count
4.2 Total Aerobic Plate Count
CHAPTER FIVE
5.1 Discussion
5.2 Conclusion
5.3 Recommendations
REFERENCES
LIST OF TABLES
4.1 Microbial Load of the Air from Laboratory of Collection
4.2 Total Coliform count for Chemistry Laboratory and Microbiology Laboratory of Olabisi Onabanjo University
4.3 Total Aerobic Plate Count for Chemistry Laboratory and Microbiology Laboratory of Olabisi Onabanjo University
4.4 Cultural Characteristics of Bacterial Isolate
4.5 Biochemical Reaction of Bacterial organisms Isolated from Laboratory
4.6 Bacteria Isolated from the Laboratories
4.7 Characteristics of Fungal Isolates

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