Milk and Milk products derived from dairy cow can harbour microorganisms and can serve as sources of foodborne pathogens. This study was carried out to determine the microbial loads and prevalence of Salmonella species in milk drinks marketed in Ijebu-ode, Ogun State. The microbial load was determined by serial dilution and spread plate techniques while the isolation and identification of Salmonella species was done using Salmonella Shigella agar and conventional biochemical methods. Four out of the nine products sampled had no microbial growth while the remaining five had total viable Salmonella count that ranged from 7.0 x 105 cfu/ml to 6.3 x 106 cfu/ml., with BBStar having the highest microbial load while Tropical drink has the lowest microbial load. Five species of Salmonella were isolated out of which three were Salmonella typhi and two were Salmonella paratyphi. The high microbial load and isolation of Salmonella species in these pasteurized milk drinks pose a health risk to consumers especially children.
TABLE OF CONTENTS
CHAPTER ONE
1.1 Introduction
1.2 Aim of the Study
1.3 Specific Objectives
CHAPTER TWO: LITERATURE REVIEW
2.1 Salmonella spp.
2.2 Growth and survival characteristics
2.3 Microbiological Quality of Pasteurized Milk
2.4 Factors Affecting Microbiological Quality of Pasteurized Milk
CHAPTER THREE: MATERIALS AND METHOD
3.1 Collection of Samples
3.2 Sterilization of Glass wares
3.3 Preparation of Media
3.4 Isolation of Salmonella species
3.5 Identification and Characterization of Salmonella spp.
3.5.1 Microscopic Identification of Bacteria Isolates Gram Staining
3.5.2 Indole Test
3.5.3 Motility Test
3.5.4 Oxidase Test
3.5.5 Urease Test
3.5.6 Citrate Test
3.5.7 Sugar Fermentation Test
CHAPTER FOUR: RESULTS
CHAPTER FIVE
5.1 Discussion
5.2 Conclusion and Recommendations
Reference
LIST OF TABLES
3.1 Code of samples
4.1 Showing the total viable count of the milk samples
4.3 Morphological and biochemical characteristics of isolates recovered from milk sample
4.3 Frequency and percentage of occurrence of isolated organisms from milk samples
