ISOLATION AND CHARACTERIZATION OF BACTERIA FROM THREE RIVERS IN AGO- IWOYE, OGUN STATE.

Aquatic environment receives a significant number of human microbial pathogens from point and non-point source of pollution. Rivers is not left out of the aquatic environment and water from river is a vital natural resources because of its basic role to life. This study was carried out to investigate the microbial quality and bacteria isolates in three rivers in Ago-Iwoye, Ogun State. Six water samples were collected from the beam and middle part of three different rivers and transported to the laboratory for microbial analysis. The water samples were investigated for the presence of indicator organisms such as total coliforms, Escherichia Coli, StaphylococousAureus and Pseudomonas Aeruginosausing the spread plate method.StaphylococousAureushad the highest frequency of distribution of 53%, Pseudomonas Aeruginosahad 33.3%, while Escherichia Coli had 16.7%.It was concluded that river water used by residents of Ago-Iwoye, Ogun State for drinking, domestic purposes and agricultural uses are of poor microbial quality. Therefore river water should be adequately treated before consumption and residents should also improve on their general hygiene practices.

TABLE OF CONTENTS

CHAPTER ONE: INTRODUCTION

• Background of the study
• Statement of problem
• Aim and objectives of the study
• Specific objectives area
• Justification of the study
• Limitation of study .

CHAPTER TWO: LITERATURE

• Detection of bacterial pathogens in river water
• Bacterial pathogenesis in the aquatic environment
• Methods used in detection of bacterial pathogens in water

CHAPTER THREE

• Collection of Samples
• Preparation of Media
• Serial Dilution and Inoculation of Media
• Characterization of Isolates
• Biochemical Test for Identification of Microorganisms

CHAPTER FOUR

4.1    Frequency and percentage of occurrence of isolates from sample

4.2    Total viable bacterial, total coliform, total staphylococcusaureus count

4.3    Characterization and identification of isolates

CHAPTER FIVE

• Discussion
• Conclusion and Recommendation

REFERENCES