ISOLATION AND CHARACTERIZATION OF ACTINOMYCETES FROM AGRICULTURAL SOIL SAMPLES

ISOLATION AND CHARACTERIZATION OF ACTINOMYCETES FROM AGRICULTURAL SOIL SAMPLES

Project Details

ISOLATION AND CHARACTERIZATION OF ACTINOMYCETES FROM AGRICULTURAL SOIL SAMPLES



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Description

ABSTRACT
Actinomycetes are group of bacteria, which are present widely in ecological diversity. Actinomycetes are the major microbial population in soil which can produce active secondary metabolites. Aim of this study is to isolate and characterize varieties of actinomycetes from different agricultural soil. Soil samples were collected from different agricultural location (Vegetable soil, Maize soil, Cochorus soil, Banana soil, Cocoyam soil, Swampy soil, Stubborn grass soil and Forest soil) and used for actinomycetes isolation. The twenty-five actinomycetes classified into Streptomyces and Actinobacteria isolates were isolated from different soil samples based on their colony morphology. The isolates were analysed for their biochemical activity and found that all the isolates were positively active for catalase, motility and urease.
Further study into the utilization of the actinomycetes for agriculture industry needs to be done to fully utilize these potential microbes for sustainable agriculture.
TABLE OF CONTENTS
CHAPTER ONE
1.1 Introduction
1.2 Statement of the Problem
1.3 Justification of Study
1.4 Aim of the Study
1.5 Objectives of the Study
CHAPTER TWO: Literature Review
2.1 General characteristics of actinomycetes
2.2 Distribution of actinomycetes in natural habitats
2.2.1 Actinomycetes in plant rhizosphere soils
2.3 Isolation of soil actinomycetes
2.3.1 Isolation media
2.3.2 Physical or chemical pretreatment method
2.3.3 Other method for selective isolation of soil actinomycetes
2.4 Identification and classification of actinomycetes
2.5 Actinomycetes as biological control agents
CHAPTER THREE: Materials and Methods
3.1 Collection of Soil samples
3.2 Materials and Apparatus
3.3 Isolation of Actinomycetes
3.4.2 Biochemical Characterization
CHAPTER FOUR: Results
CHAPTER FIVE
5.1 Discussion
5.2 Conclusion
References

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