ABSTRACT
Indoor air quality is gaining importance in residential sector whilst it is the need of hours to assess indoor air quality in school laboratories with respect to indoor air microflora because of the infection that can be transmitted through air. The study is therefore aimed at the isolation and characterization of aerosolic bacteria and fungi from different laboratory environments in the Faculty of Science, Olabisi Onabanjo University, Ago-Iwoye. The air sample was collected by exposing prepared solidified nutrient agar and potato dextrose agar for the enumeration and isolation of bacteria and fungi respectively. The total bacteria counts were reported as the number of CFU per cubic meter. The bacteria counts of air samples ranged from 6 to 144 cfu/m3. Bacteria isolated from the laboratories were Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and Micrococcus luteus. Fungi isolated were Aspergillus niger, Aspergillus nidulans and Rhizophores stolonifera. Out of the thirty bacteria isolated in this study, fifty-three (53%) were susceptible to Septrin, followed by Ciprofloxacin and Streptomycin with 47%, Erythromycin and Pefloxacin with 40%, Gentamycin with 23%. The organisms were least susceptible to Ampiclox, Zinnacef and Amoxicillin with 10% each. Hence, proper disinfection practice, working under a standard sterile condition, quality monitoring of air and maintenance of devices that can transmit bioaerosol across different locations are highly recommended.
TABLE OF CONTENTS
CHAPTER ONE
1.1 Introduction
1.2 Aim of the Study
1.3 Objectives of the Study
CHAPTER TWO: Literature Review
2.1 Antibiotic Resistance
2.2 Prevalence of Bacteria and Fungi in Indoor Environment
2.3 Bioaerosol and Transmission of Diseases
2.4 Sources of Airborne Bacteria and Fungi
CHAPTER THREE: Materials and Methods
3.0 Study Area
3.1 Collection of Samples
3.2 Instrument and glass wares
3.3 Media Used
3.4 Method of Sterilization
3.5 Isolation of Microorganisms
3.6 Characterization of Bacterial Isolates
3.6.1 Gram’s Staining Reaction
3.6.2 Catalase
3.6.3 Oxidase Test
3.6.4 Indole Test
3.6.5 Citrate Test
3.6.6 Coagulase Test
3.6.7 Sugar Fermentation Test
3.7 Identification of Fungi
3.8 Antibiotic Susceptibility Testing for Bacterial Isolates
3.8.1 Preparation of Inoculum
CHAPTER FOUR: Results
CHAPTER FIVE: Discussion, Conclusion and Recommendations
5.1 Discussion
5.2 Conclusion
5.3 Recommendations
References
