This study aimed to determine the prevalence and antibiotic susceptibility patterns of extended spectrum beta lactamase (ESBL) producing Escherichia coli isolates isolated from faecal samples of apparently healthy chickens in four poultry farms in Ago Iwoye Ogun State. Isolates were identified by relevant biochemical tests. The antibiotics susceptibility testing were determined for five(5) antibiotics (gentamicin, cefuroxime, erythromycin, ceftazidime and ceftriaxone).Test for ESBL production was carried out by the double disk diffusion method using amoxicillin clavulanic (30µg), ceftazidime (30µg) and cefuroxime (30µg) alone E. coli was isolated in 8 of 60 samples examined from which they were all beta lactamase positive as they confer resistance to the antibiotics used. E.coli exhibited high resistance to cefuroxime, erythromycin, augmentin, ceftazidime and ceftriaxone in all the isolates.98% of the isolates were susceptible to gentamicin and 45% to erythromycin as zone of inhibitions were seen and recorded. In conclusion, the study has demonstrated the presence of E.coli isolates in various faecal sample of chickens in low concentration, their antibiotic susceptibility clearly demonstrating multiple drug resistance and the prevalence of extended spectrum beta lactamase in some isolates.
TABLE OF CONTENT
CHAPTER ONE
1.0 Introduction
CHAPTER TWO: LITERATURE REVIEW
2.0 Escherichia coli
2.1 Beta Lactams
2.2 Beta Lactamases
2.3 Evolution and epidemiology of extended-spectrum beta-lactamase
2.3.1 Integrons: Classes of Integrons
2.4 Definition and classification of extended-spectrum beta-lactamases
2.4.1 Definition
2.4.2 Classification
2.4.3 Risk factors associated with the acquisition of ESBL- producing Enterobactericeae
2.5 Treatment of patients infected with Enterobacteriaceae producing ESBLs (ESBLA)
CHAPTER THREE
3.0 MATERIALS AND METHODS
3.1 Collection and processing of specimen
3.2 Sterilization of materials used
3.3 Isolation and identification of organisms
3.3.1 Culture media
3.3.2 Media preparation
3.3.3 Microbiological analyses
3.3.4 Biochemical tests
3.3.4 Identification method
3.4 Susceptibility testing
3.5 Test for ESBL production
CHAPTER FOUR
4.0 RESULTS
CHAPTER FIVE
5.0 DISCUSSION
5.1 CONCLUSION
REFERENCES
