The frequency of bacteria in raw and processed cattle hides in Ijebu – Igbo, Ogun State, was examined in this study. Hundred samples of cattle hides including raw (40), processed (soaked) (30), processed (unsoaked) (30) were collected. The raw and processed (soaked) were collected from different market women while the processed (unsoaked) was collected from cattle hide processors at different locations and were analyzed microbiologically. Out of 100 samples analysed, 74 (74%) were contaminated with bacteria of different spieces including: E. coli (18 %) which had the highest occurrence, followed by B. cereus (12%), S. enteric (7%), P. aeruginosa (7%), S. aureus (7%), B. subtilis (5%), V. parahaemoliticus (4%), S. pneumonia (4%), A. hydrophila (2%), E. faecalis (2%), K. oxytoca (2%), P. vulgaricus (2%) and K. pneumonia (2%). E. feacalis, P. aerumonas, V. parahaemolyticus, B. subtilis, B. cereus, S. enterica, S. aureus and E. coli were found in both raw and processed (soaked) samples. P. aeruginosa, B. cereus, S. aureus and E. coli were found in the three samples. S. pneumonaie was found in both processed (unsoaked) and (processed soaked). P. vulgaricus was found in only raw. K. oxytoca was found only processed (unsoaked). A. hydrophila and K. pneumonaie were found only in processed (soaked). Antimicrobial susceptibility of isolated microorganism were determined using the Kirby and Bauer method of disk diffusion. The antibiotics used are: tetracycline, gentamicin, doxycilin, amoxicillin, streptomycin, ciprofloxacin, chloramphenicol and augmentin. Most of the isolates were susceptible to most of the antibiotics used. Only a few showed resistance. E. faecalis showed resistance to doxycilin, P. aeruginosa to augmentin and ciprofloxacin, A. hydrophila to ciprofloxacin, B. cereus to amoxicillin and doxycilin, and E. coli to augmentin. This result confirmed that bacterial contaminants in cattle hides in Ijebu – Igbo are still susceptible to antimicrobial treatment despite their wide prevalence.
TABLE OF CONTENT
CHAPTER ONE
1.0 Introduction
1.1 Aims and objective
CHAPTER TWO
2.0 Literature review
2.1 Cattle hides and their sources of contamination
2.2 Bacteria often associated with cattle hides
2.3 Antimicrobial susceptibility in cattle hides
2.4 Decontamination of cattle hides
CHAPTER THREE
3.0 Materials and method
3.1 Collection of samples
3.2 Sterilization of glasswares
3.3 Media composition
3.4 Media preparation
3.5 Sampling of cattle hides
3.6 Isolation of microorganisms
3.7 Identification and characterization of isolated organisms
3.8 Biochemical characterization
3.9 Antibiotic susceptibility testing
CHAPTER FOUR
4.0 Results
CHAPTER FIVE
5.0 Discussion
5.1 Conclusion
REFERENCES
LIST OF TABLES
1 Microbial load in cattle hides for 10-2 dilutions (103)
2 Microbial load in cattle hides for 10-4 dilutions (105)
3 Morphological characterization of isolates
4 Biochemical characterization of isolates
5 Percentage of contaminated cattle hides
6 Frequency distribution of isolated bacteria from examined cattle hides
7 Comparison between microorganisms gotten from each sample
8 Antimicrobial susceptibility test
