COMPARATIVE STUDY ON MICROBIAL LOAD ISOLATED FROM DIFFERENT WATER FILTERATION UNITS USED IN INDUSTRIES

COMPARATIVE STUDY ON MICROBIAL LOAD ISOLATED FROM DIFFERENT WATER FILTERATION UNITS USED IN INDUSTRIES

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COMPARATIVE STUDY ON MICROBIAL LOAD ISOLATED FROM DIFFERENT WATER FILTERATION UNITS USED IN INDUSTRIES



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ABSTRACT
Filtration is one of the most important separation processes that can be employed to remove deadly organisms from water in industries. Even though, it has been used over a century, there is still need to continue to research on water from filtration unit in order to prevent outbreak of diseases via beverages and water produced from industries. The aim of the study is to compare the microbial load from different water filtration units used in industries. Sterile containers and swab sticks were used to collect thirty-six samples from three filtration plants: 12 raw water sample, 12 swab sample from the bed filter and 12 filtered water sample. The microbial and biochemical analysis were also determined using standard methods. The pH of the water samples ranged from 6.2 to 8.1. The temperature of the water samples ranged from 20.7oC – 27.7oC. Microbial count from the water sample ranged from 48 cfu/ml to 208 cfu/ml. It was observed that there was no growth in all the samples collected after treatment. The total microbial count from the swab samples ranged from 178 cfu/ml to 298 cfu/ml. A total number of twenty-one bacteria belonging to six genera were isolated from the sample collected. The isolated organisms from the water sample include Klebsiella pneumoniae Staphylococcus aureus, Pseudomonas aureus, Enterobacter sp. and Chomobacterium violaceum while the organisms isolated from the bed filter include Enterobacter sp., Klebsiella pneumonae, Staphylococcus aureus and Escherichia coli. It is recommended that the sources of water in industries should be examined frequently in order to continue to have confidence on the water used for production.
TABLE OF CONTENTS
CHAPTER ONE
1.0 Introduction
1.2 Significance of the Study
1.3 General Objective
1.4 Specific Objectives
CHAPTER TWO: Literature Review
2.1 Water and Filtration in Industries
2.2 Purpose of Water Filtration
2.3 Water Quality Parameters and Standard
2.4 Filtration
2.5 Types of Filtration
2.6 Efficacy and Evaluation of Filters for Industrial Water Treatment
CHAPTER THREE: Materials and Methods
3.1 Sample Collection
3.2 Sample Site
3.3 Materials Used
3.4 Sterilization of Glasswares
3.5 Media Used
3.6 Media Preparation
3.7 Physical and Chemical Analysis of the Water Samples
3.8 Microbial Analysis
3.8.1 Coliform count (TCC)
3.8.2 Total Viable Count Determination
3.8.3 Culture Techniques
3.8.4 Morphological Characteristics
3.8.5 Gram Staining
3.9.3 Biochemical Characterization
CHAPTER FOUR: Results
CHAPTER FIVE: Discussion, Conclusion and Recommendations
5.1 Discussion
5.2 Conclusion
5.3 Recommendations
References

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