Abstract
Escherichia coli is one of the most frequent pathogenic bacteria isolated from urine samples and they are able to attach to the bladder wall and form a biofilm that contribute to virulence of bacteria which resist the body’s immune response. This study was conducted to assess Escherichia coli from urine of university students for biofilm production. 60 urine samples were collected from students of Olabisi Onabanjo University into a sterile universal bottle and transported to the Department of Microbiology laboratory for immediate culture on Eosin Methylene Blue agar. The bacteria isolates were examined and characterized biochemically using standard microbiological method. E. coli was found to be the most frequent isolated pathogen (n=12, 20%), followed by Klebsiella spp. (n=10, 16.6%), E. coli / P. aeruginosa (n=6, 10%), Pseudomonas aeruginosa (n=5, 8.3%) and Proteus mirabilis (n=3, 5%). Out of the Eighteen (18) E. coli subjected to biofilm production, 16 (88.8%) isolates produced biofilm by TCP method and 13(72.2%) produced biofilm by Tube Method. Presence of this pathogen in urine tract may lead to multiplication and establishment of urinary tract infection which females are more prone to than males. The ability of E. coli to form a biofilm which resists the body’s immune response may pose a public health problem for the host patients as the microorganisms in biofilm contribute to virulence of bacteria that are difficult to treat with antimicrobial agents. Good knowledge and proper education should be made known to the students and general populace on urinary tract infections and the causes.
TABLE OF CONTENTS
CHAPTER ONE
1.1 Introduction
1.2 Aims and Objectives
1.3 Objective of the study
1.4 Literature Review
CHAPTER TWO: Materials and Methods
2.1 Samples Collection
2.2 Sterilization of Materials
2.3 Preparation of Culture Media
2.4 Isolation of Escherichia Coli
2.5 Characterization and Identification of Bacteria
2.5.1 Cultural and Morphological Characteristics
2.5.2 Gram Stain Reaction
2.6 Identification Methods
2.6.1 Urease Test
2.6.2 Indole Test
2.6.3 Oxidase Test
2.6.4 Catalase Test
2.6.5 Citrate Utilization Test
2.6.6 Motility Test
2.6.7 Voges-Proskauer (VP) Test
2.7 Biofilm Formation Assay of Escherichia coli
2.7.1 Test Tube Method
2.7.2 Tissue culture Plate Method
CHAPTER THREE
3.1 Results
CHAPTER FOUR: Discussion and Conclusion
4.1 Discussion
4.2 Conclusion and Recommendations
References
