ABSTRACT
Industries are the major sources of pollution and dissemination of antibiotics resistance microorganisms. Hence this study was conducted to investigate the antibiotic resistance of microorganisms isolated from industrial waste dumpsites in Ibadan, Oyo state. Twelve (12) waste samples were collected, 6 from soil waste disposal site and 6 from water waste disposal sites. Isolation of bacteria was done by spread plate method. A total of thirty-five bacteria were recovered from the waste water and soil samples and characterized as Staphylococcus aureus (28.6%), Escherichia coli (25.7%), Bacillus spp. (22.9%) and Campylobacter spp (22.9%). Fungi isolated from the waste water and soil samples were candida spp. Microsporium spp. (19), Tricophyton sp. (4.8), Penicillium spp. (38.1), Aspergillus flavus (4.8), Rhizopus spp. (9.5), Aspergillus niger (9.5), Aspergillus funmigatus (4.8) Candia spp, (4.8) and Fusarium sp. (4.1). Campylobacter spp. were resistant to amoxicillin and zimacef. Human infections caused by these microorganisms may be difficult to treat with available antibiotics.
TABLE OF CONTENTS
CHAPTER ONE
1.1 INTRODUCTION
1.2 AIM OF THE STUDY
1.3 OBJECTIVES OF THE STUDY
CHAPTER TWO: LITERATURE REVIEW
2.1 Industrial Wastes
2.2 Sources of Industrial Wastes
2.2.1 Liquid Wastes in Industry
2.2.2 Solid Wastes in Industry
2.3 Ground and Surface Water Contamination by Industrial Waste
2.4 Historical Perspective on Antimicrobial Resistance
2.5 Antimicrobial Resistance in the Environment
2.6 The Burden of Antimicrobial Resistance
CHAPTER THREE: MATERIALS AND METHODS
3.1 Study area
3.2 Collection of samples
3.3 Sterilization of Glassware
3.4 Isolation and Characterisation of Organisms
3.5 Morphological Characterization
3.5.1 Gram staining
3.6 Biochemical Tests
3.6.1 Indole production test
3.6.2 Coagulase test
3.6.3 Catalase test
3.6.4 Oxidase test
3.6.5 Citrate utilization test
3.6.6 Urease Test
3.7 Antimicrobial Resistance Testing
3.8 Determination of Multiple Antibiotic Resistances
CHAPTER FOUR: RESULTS
CHAPTER FIVE: DISCUSSION AND CONCLUSION
5.1 Discussion
5.2 Conclusion
REFERENCES
