47 Pages, Chapter 1-5
MS Word
08062235854
The significance of Escherichia coli as an indicator of water contamination is well established. The isolation and antibiotic resistance profile of Escherichia coli present in well water located within public primary schools was carried out using standard microbiological methods. Isolation was done by spread plate method on Eosin methylene blue agar. The total coliform count and viable countof the samples were done by pour plate method on MacConkey agar and Nutrient agar plates respectively. The antimicrobial susceptibility of isolates to eight (8) antibiotics was done using disc diffusion method. A total of 40 Escherichia coli were isolated. Total coliform count of the water samples ranged from 0.92 × 103 CFU/ml to 2.89 × 103 CFU/ml and the total viable count of the water samples ranged from 0.50 × 103 CFU/ml to 2.34 × 103CFU/ml. Isolates showed varying degree of resistance the highest being Ampicillin 87.5%, Amoxicillin 54.7%, Nalidixic acid 51.5%, Streptomycin 15.5%, Chloramphenicol 75.5%, Tetracycline 62. 5%. 15 of the well water isolates were found to be multidrug resistance (MDR). These result shows that the sampled well water served as reservoir of Escherichia coli which is of great consequences to public health, especially the school children.
TABLE OF CONTENTS
CHAPTER ONE: INTRODUCTION
1.1 Background to the Study
1.2 Aim of the Study
1.3 Objectives of the Study
1.4 Justification
CHAPTER TWO: LITERATURE REVIEW
2.1 Microbiology of E. coli
2.1.1 Epidemiology of E.coli
2.1.2 Reservoirs of E. coli
2.2 Waterborne Transmission of Enteric Bacteria
2.3 Prevalence in Surface Water
2.4 Pathogenic Bacteria and Indicator Bacteria
2.5 Survival of Enteric Bacteria in Water
2.5.1 Effect of Temperature
CHAPTER THREE: MATERIALS AND METHOD
3.1 Selection of Sampling Area
3.2 Collection of Samples
3.3 Material and Apparatus Used
3.4 Sterilization of Glasswares
3.5 Media Used
3.6 Media Preparation
3.7 Determination of Microbial Load
3.8 Isolation of Escherichia coli
3.9 Purification of Isolates
3.10 Identification and Characterization of Escherichia coli
3.10.1 Microscopic Identification of Bacteria Isolates Gram Staining
3.10.2 Catalase Test
3.10.3 Oxidase Test
3.10.4 Citrate Utilisation Test
3.10.5 Urase Test
3.10.6 Indole Test
3.10.7 Motility Test
3.10.8 VogesProskauer Test
3.10.9 Methyl Red Test
3.11 Antibiotic Susceptibility Test
3.12 Preparation of Inoculum
3.13 Determination of Multiple Antibiotic Resistances
CHAPTER FOUR: RESULTS
4.1 Observations made on Sites
4.2 Frequency and percentage of occurrence of Escherichia coli from well water samples
4.3 Morphological and biochemical characteristics of Escherichia coli
4.4 Total Coliform Count
4.5 Total viable count
4.6 Antimicrobial susceptibility testing
CHAPTER FIVE
5.1 Discussion
5.2 Conclusion
5.3 Recommendations
REFERENCE
