MOLECULAR ANALYSIS OF Apis mellifera adansoni FROM THE ENVIRONMENT OF IJEBU IN OGUN STATE, NIGERIA

This study examined the molecular analysis of Apis mellifera adansonii from the environment of Ijebu in Ogun State, Nigeria. Honeybee samples were collected from 22 hives/colonies and subjected to molecular analysis using SSR markers to determine the genetic variability of the honeybees. The extracted DNA were of good quality and were extracted using modified Murray and Thompson method. The DNAs were amplified using 7 SSR primers; A81, A29, A113, A107, A14, IC1, B124. The bands were scored for their presence (1) or absence (0) in the SSR profile populations to determine the genetic diversity and to construct the dendogram. Factorial analysis showed that there is genetic differentiation in the populations of honey bees. In all, 81bands were scored out of which 80 were polymorphic and only one band was monomorphic.

TABLE OF CONTENTS

CHAPTER ONE: Introduction

1.1       The honeybee

1.2       Species and location of honeybee

1.3       Products of honeybee

1.4       Aims and objectives

CHAPTER TWO: Literature review

2.1       Origin of biodiversity of Apis mellifera                                             

2.2       Morphological analysis of Apis mellifera

2.3       Genetic diversity of honeybee

2.3.1    DNA based markers for genetic diversity study

2.3.2    The PCR based methods

2.3.3    The non-PCR based methods

CHAPTER THREE: Materials and Methods

3.1       Materials

3.2       Methods

3.2.1    Sampling and DNA extraction

3.2.2    Dilution of primers

3.2.3    Amplification

3.2.4 Data analysis

CHAPTER FOUR                                                                                                

4.1 Results

4.1.1 Factorial analysis

CHAPTER FIVE:    Discussion and Conclusion

5.1 Discussion

5.2 Conclusion

REFRENCES                                                                                              

APPENDIX