ISOLATION OF CELLULOSE FROM SAND BOX (Hura crepitans) SEED SHELL

ISOLATION OF CELLULOSE FROM SAND BOX (Hura crepitans) SEED SHELL

Project Details

ISOLATION OF CELLULOSE FROM SAND BOX (Hura crepitans) SEED SHELL



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Description

ABSTRACT

Cellulose is an important structural component of primary cell wall in green plant and about billion tons of cellulose is being produced from various plants. Cellulose is renewable, thermally stable, cheap, light in weight and the chemical formula (C6H10O5)n.

The cellulose was isolated by delignification process and alkaline hydrolysis. Percentage yield and proximate analysis were determined. It was characterized by FTIR, SEM and XRD.

The percentage cellulose yield was 10.14%.  Fibre content of isolated Hura crepitans seed shell (1.68%) was observed to be lower than that for the isolated cellulose (3.11%). The moisture content of the isolated Hura crepitans seed shell (11.87%) was observed to higher than that of the raw Hura crepitans seed shell (11.79%). The percentage ash content (2.65%) of isolated Hura crepitans seed shell was also lower than that of raw (4.68%). Percentage crude fat (2.97) of isolates was lower than that of raw sample (2.18%). The broad band in the region 500-4500 cm-1 is due to O-H stretching vibration and it gives considerable information of the hydrogen bonds.

This study shows that Hura crepitans seed shell contains cellulose which is crystalline in nature.

TABLE OF CONTENTS

CHAPTER ONE

1.1       Introduction

1.2       Aim and Objectives of the Study

CHAPTER TWO

2.0       Literature Review

2.1       Cellulose

2.1.1    Cellulose as a Polysaccharides

2.1.3    General structure of cellulose

2.1.4    Cellulose microfibril organization

2.1.5    Diffraction principle

2.2       Hura crepitans                                                                                                          

CHAPTER THREE

3.0       Materials and Methods

3.1       Materials

3.1.1    Materials Sample Collection

3.1.2    Apparatus

3.1.3    Reagent

3.2       Methods

3.2.1    Isolation of Cellulose

3.2.2    Percentage Yield of Cellulose

3.2.3    Characteristics of Cellulose

3.3.3    Diffractometer technique

3.3.4    Sample preparation

CHAPTER FOUR

4.0       Results and Discussion

CHAPTER FIVE: Discussion and Conclusion

5.1       Summary

5.2       Conclusion

5.3       Recommendation

Appendix

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