ISOLATION AND SCREENING OF SOIL BACTERIA FOR POTENTIAL ANTIMICROBIAL ACTIVITY

ISOLATION AND SCREENING OF SOIL BACTERIA FOR POTENTIAL ANTIMICROBIAL ACTIVITY

Project Details

ISOLATION AND SCREENING OF SOIL BACTERIA FOR POTENTIAL ANTIMICROBIAL ACTIVITY



Contact Chris Research

Description

ABSTRACT
Antibiotic is one of the most important commercially exploited secondary metabolites produced by bacteria and employed in a wide range. Most of the antibiotics used today are from the microorganisms. This study was conducted to isolate and screen soil bacteria for antibiotic production. Soil samples were collected from different fruit and vegetable farms in Ago-Iwoye, Ogun State and isolation of bacteria was done by spread plate method using Nutrient agar after serial dilution. The total viable counts of the samples ranged from 1.9 x 106 cfu/g to 7.0 x 106 cfu/g. Fourteen (14) isolates belonging to five genera were isolated in this study. They were Escherichia coli (4), Staphylococcus aureus (3), Klebsiella pneumoniae (3), Streptococcus pneumoniae (2) and Pseudomonas aeruginosa (2). Five out of these isolates, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Streptococcus pneumoniae and Pseudomonas aeruginosa were screened for antibiotic production. Escherichia coli inhibited the growth of Klebsiella pneumoniae while Klebsiella pneumoniae inhibited the growth of Pseudomonas aerugionosa. However, Streptococcus pneumoniae, Staphylococcus aureus and Pseudomonas aerugiona did inhibit growths of the test organisms. The results of this study indicate that the potential of some soil bacteria produce antimicrobial compounds that can be useful for many applications and it must be better explored.

TABLE OF CONTENTS
CHAPTER ONE
1.0 Introduction
1.1 Aim of the Study
1.2 Objectives of the Study
CHAPTER TWO
2.0 Literature Review
2.1 Some Bacteria that have Ability to Produce Antibiotics
2.1.1 Bacillus spp.
2.1.2 Actinomycetes
2.1.3 Streptomyces
CHAPTER THREE
3.0 Materials and Methods
3.1 Collection of Samples
3.2 Materials Used
3.3 Medium Used
3.4 Preparation of Media
3.5 Isolation of Microbes
3.6 Characterization and Staining Methods
3.7 Biochemical Test
3.7.1 Indole Test
3.7.2 Catalase Test
3.7.3 Citrate Utilization Test
3.7.4 MR-VP Test
3.7.5 Triple Sugar Iron Agar Test (TSI)
3.8 Test Bacteria
3.9 Screening of Soil Isolates for Antibiotic Activity
CHAPTER FOUR: RESULTS
CHAPTER FIVE: DISCUSSION AND CONCLUSION
5.1 Discussion
5.2 Conclusion
REFERENCES

Leave a Reply

Your email address will not be published. Required fields are marked *