Milk is nutritious and an essential food for man as well as a good medium for microbial growth and contamination. The study was designed to determine the microbial and prevalence of Staphylococcus species in pasteurized milk drinks marketed in Ijebu- ode, Ogun state. The microbial loads in these milk drinks were determined and the isolation and identification of Staphylococcus species was done on Manitol Salt Agar using spread plate technique and the conventional biochemical methods. Five out of the nine products analyzed had bacteria growth while the remaining four had no growth. Total viable counts observed from the five products with bacterial growth ranging from 4.0 x 105 to 1.5 x106 cfu/ml with Cway milk drink having the highest microbial load. A total of five Staphylococcus species were isolated out of which three were S. aureus and two were S. epidermis. The high microbial loads and isolation of Staphylococcus spp. in these pasteurized milk drink pose a health risks to consumers.
TABLE OF CONTENT
CHAPTER ONE
1.1 Introduction
1.2 Aim and Objective of the Study
1.3 Specific Objectives
CHAPTER TWO: Literature Review
2.1 Health Benefit of Milk
2.2 Pasteurization
2.2.1 Purpose of Pasteurization
2.2.2. Methods of Pasteurization
2.3 Staphylococcus aureus
2.3.1. Virulence characteristics of S. aureus
2.3.2. Toxins
2.4 Staphylococci and Food Poisoning
2.5 Occurrence of Staphylococcus in Milk Products
CHAPTER THREE: Materials and Method
3.1 Collection of Samples
3.2 Sterilization of Glasswares
3.3 Preparation of Media
3.4 Isolation of Staphylococcus species
3.5 Identification and Characterization of isolates.
3. 5.1 Gram Staining
3.5.2 Catalase Test
3.5.3 Coagulase Test
3.5.4 Oxidase Test
3.5.5 Sugar Fermentation Tests
3.5.6 DNASE Test
CHAPTER FOUR: Results
4.1 Identification of Staphylococcus spp.
CHAPTER FIVE: Discussion, Conclusion and Recommendations
5.1 Discussion
5.2 Conclusion and Recommendations
References
LIST OF TABLES
3.1 Code of the samples
4.1 Total Viable Count of pasteurized the Milk product marketed in Nigeria
4.2 Morphological and Biochemical characterization of Staphylococcus species isolated from milk products
4.3 Frequency and Percentage of Occurrence of Isolated Organisms from Milk Samples
