ABSTRACT
Lipolytic enzymes are currently attracting significant attention because of their biotechnological potential. Most of the lipases used in industry are microbial enzymes, of both fungal and bacterial origin. The present study deal with isolation and characterization of lypolytic fungi from the atmosphere. Prepared Rhodamine B and Tributyrin agar were exposed to the atmosphere in the Microbiology Laboratory of Olabisi Onabanjo University for 15 – 30 minutes. Screening of lipase producing fungi was isolated using Rodamine B and tributyrin as a substrate on agar plates. A total of nine (9) lipolytic organisms was recovered from the environment. The isolated organisms includes Aspergillus niger, Aspergillus carbonarius, Abisidia spp., Aspergillus flavus, Aspergillus niger had the highest percentage with 4(44.5%), followed by Apsergillus flavus and Absida spp. with 2(22%) and 2(22%) respectively. The result of this study showed that lipase activity microorganisms can be isolated by exposing rhodamine B agar and tributyrine agar to the air. Lipases are ubiquitous enzymes of considerable physiological significance and industrial potential. So studies to analyze the production of the enzyme from microbial source are essential from the research, environmental and commercial point of view.
TABLE OF CONTENTS
CHAPTER ONE
1.1 Introduction
1.2 Aim and Objective of the Study
1.2.1 Aim of the Study
1.2.2 Objectives of the Study
1.3 Significance of the Study
CHAPTER TWO
2.1 Lipases
2.2 Sources of Lipases
2.3 Microbial Lipases
2.3.1 Fungi as Source of Lipase
2.3.2 Lipase assay for fungal culture
2.4 Isolation of lipase-producing microorganisms
2.5 Lipases Production
2.6 Applications of Lipases
2.6.1 Food, Fat and oil Industry
2.6.2 Dairy Industry
2.6.3 Bakery Industry
2.6.4 Lipases in the detergents
2.6.5 Lipases in pulp and paper industry
2.6.6 Lipases in cosmetics and perfumery
2.6.7 Lipases in leather industry
2.6.8 Lipases in bioremediation
2.6.9 Lipases as biosensors
2.7 Methods for the Screening of Lipase Producing Microorganisms
2.7.1 Qualitative Methods
2.7.1.1 Gel Diffusion assay using different substrates (lipidic)
2.7.1.2 Gel Diffusion assay with indicator dyes
2.7.2 Quantitative Methods
2.7.2.1 Titrimetric Methods
2.7.2.2 Colorimetric Methods
2.7.2.3 Colorimetric methods using p-nitrophenyl esters
2.7.2.4 Radial diffusion method
2.7.2.5 Fluorescence assay
2.7.2.6 Chromatographic methods
CHAPTER THREE: Materials and Methods
3.1 Media Used
3.2 Collection of Samples
3.3 Characterization of Lipase Producing Organisms
3.4 Screening of lipolytic fungal species
CHAPTER FOUR: Results
CHAPTER FIVE
5.1 Discussion
5.2 Conclusion
References
