40 Pages, Chapter 1-5
MS Word
08062235854
Bananas are colonized by bacteria, fungi and other agents causing different types of disease. Banana, due to their low pH, high moisture content and nutrient composition are highly susceptible for the colonization of pathogenic bacteria and fungi. This study investigated the microorganisms associated with the spoilage of banana fruits in Ago-Iwoye, Ogun State. As part of food quality surveillance study, this work looked at the incidence of bacteria associated with banana fruits Ago-Iwoye, Ogun State. Two samples of banana (Musa sapientumand Musa acuminatavar. dwarf Cavendish) were purchased from the market in Ago-Iwoye, Ogun State. The total coliform counts were determined by the spread plate method on nutrient agar. There was a continuous decrease in bacterial colony count of fruits as serial dilution process progressed for the two banana samples. The microorganisms isolated were identified microscopically and by biochemical tests. Results of the microbial test showed that the bacteria isolated were Streptococcus sppand Proteus spp(from Musa acuminatavar. dwarf Cavendish) and Alcaligenessppand Streptococcus sppwere isolated from Musa sapientum.
The results of this work showed that streptococcal isolates were most predominant compared to the other isolates.
Key words: Spoilage, Fruits, Nutrient composition, Banana, Bacteria, Colony count.
TABLE OF CONTENTS
CHAPTER ONE: Introduction
1.1 Background To The Study
1.2 Aim of the Study
1.3 Objectives of the Study
CHAPTER TWO: Literature Review
2.1 Origin, Cultivation And Distribution Of Banana
2.2 Taxonomy
2.3 Botanical Description Of Banana
2.3.1 Preferred Scientific Name
2.3.2 Family
2.3.3 Common Names
2.3.4 Size
2.3.5 Flowers
2.3.6 Leaves
2.3.7 Fruit
2.3.8 Seeds
2.4 Ecology of Banana
2.5 Nutrition of Banana
2.5.1 Vitamins
2.5.2 Antioxidants
2.5.3 FibreAnd Resistant Starch
2.5.4 Bananas, Tryptophan And Serotonin
2.5.5 Potassium
2.6 Importance And Uses Of Banana
2.6.1 Canned Slices
2.6.2 Chips (Crisps)
2.6.3 Jam And Jelly
2.6.4 Medical Uses
2.6.5 Other Uses
2.6.6 Animal Feed
CHAPTER THREE: Materials and Method
3.1 Description Of Study Area
3.2 Materials
3.3 Sample Collection
3.4 Sterilization Of Apparatus
3.5 Serial Dilution
3.6 Media Preparation
3.7 Purification Of Bacteria
3.8 Gram Staining
3.8.1 Bacteria Characterization
3.8.2 Morphological Characteristics
3.9 Biochemical Tests
3.9.1 Catalase Test
3.9.2 Indole Test
3.9.3 Oxidase Test
3.9.4 Citrate Utilization Test
3.9.5 Urease Test
3.9.6 Sugar Fermentation Test
CHAPTER FOUR: RESULT
4.1 Morphological Characteristics of Cultures on Spoilt Banana Sample
4.2 Gram Stain
4.3 Biochemical Test
4.4 Microbial Count
CHAPTER FIVE
5.0 Discussion
5.1 Conclusion
References
LIST OF TABLES
Table Title Page
4.1 Morphological Characteristics of Growth on Nutrient Agar 25
4.2 Biochemical Chatracterisation of Isolates 26
4.3 Colony Counts on Nutrient Agar 27
