CHARACTERIZATION AND ANTIMICROBIAL SUSCEPTIBILITY OF BACTERIA ISOLATES FROM VEHICLE STEERING

The environments that humans encounter daily are sources of exposure to microbial communities and some are of potential concern to human health.Microbial examination of fifteen (15) vehicle steering whlees were carried out using standard methods. Thirthy-five bacteria were isolated and characterized given seven (7) genera of bacteria. The bacteria isolated with there occurrence are; Staphylococcus aureus (37%), Streptococcus spp(14%), Bacillus spp(11%), Pseudomonas aureginosa (14%), Escherichia coli (8%), Klebsiellaspp (5%), Clostridium spp (11%).The antimicrobial susceptibility result shows that Augmentin and Cloxacillin were the most resisted antibiotics, having no effect on none of the isolates exposed to them (Table 1.1), Chloramphenicol was the most effective with a 95.2% effectiveness on the organisms it was tested against, followed by Gentamicin (81%) and Tetracycline (71.4%) (Table 1.3). High percentage drug resistance was also observed for Amoxycillin (95.2%) and Cotrimoxazole (90.5%). The result of the susceptibility of antibiotics presented a varying degree of resistance to the different drugs used against the different organisms but in all, from the result of antibiotics susceptibility tests, all isolates were sensitive to at least one of the eight antibiotics they were tested against and susceptibility to five different antibiotics. Some of the organisms isolated can cause severe gastroenteritis. Regular cleaning of vehicles should be carried out.

TABLE OF CONTENTS

CHAPTER ONE                                                                                                                              Introduction

1.1       Aim of the Study

1.2       Objectives of the study

CHAPTER TWO                                                                                                    

• Literature Review

CHAPTER THREE                                                                                                

3.0       Selection of Samples

3.1       Sterilization of Glasswares

3.2       Media Preparation

3.3       Preparation of Inoculum

3.4       Isolation of Microorganisms

3.5       Identification of Isolated Organisms

3.5.1    Gram-Staining

3.5.2     Biochemical Test

3.5.5.2.1 Catalase Test

3.5.5.2.2      Oxidase Test

3.5.5.2.3      Citrate utilization test

3.5.5.2.4       Urease Activity

3.5.5.2.5       Indole test

3.6                Antibiotic Susceptibility Testing

3.6.1             Preparation of Inoculum

3.6.2             Determination of multiple antibiotic resistance

CHAPTER FOUR: RESULT

4.1       Antimicrobial Susceptibility Testing

CHAPTER FIVE                                                                                                    

Discussion

Conclusion

References