ABSTRACT
This study assessed the microbial load and spectrum on the surfaces of indigenous kitchen utensils (pestles, mortars and grinding stones). It also evaluated the rate of contamination of the indigenous kitchen utensils with bacteria, fungi and coliforms.
A total number of 60 samples were collected from surfaces of pestles, mortars and grinding stones by swabbing with sterile swab sticks from different households. The samples were collected before washing and after washing with distilled water. The samples were labelled correctly, packed in a sterile polythene bag and transported to the microbiology laboratory in Olabisi Onabanjo University, for further analysis.
The results showed that bacterial species such as Staphylococcus aureus, Escherichia coli, Klebsiella pnuemoniae, among others and were prevalent on the surfaces of the kitchen utensils.
The presence of these bacterial species could create health hazards when they come in contact with food during preparation and production. Improper handling and poor maintenance of these utensils by members of the households increase the risk of contamination.
TABLE OF CONTENTS
CHAPTER ONE: INTRODUCTION
1.1 Background to the Study
1.2 Aim and Objectives
1.3 Significance of the Study
CHAPTER TWO: LITERATURE REVIEW
CHAPTER THREE: MATERIALS AND METHODS
3.1 Materials
3.1.1 Microorganisms
3.1.2 Media
3.1.3 Chemicals
3.1.4 Equipment
3.2 Methods
3.2.1 Sample collection
3.2.2 Isolation of Microorganisms
3.2.3 Characterization and Identification of Biochemical Characteristics
3.2.3.1 Gram Staining
3.2.3.2 Lactophenol Staining
3.2.3.3 Biochemical tests
CHAPTER FOUR: RESULTS
4.1 Results
CHAPTER FIVE: CONCLUSION
5.1 Conclusion
References
