Fish culture is a relatively new enterprise in the developing world, especially in Africa. However, there are a number of constraints to its growth which include infection by bacteria. The increasing use of antibiotics in treating farm and aquatic animals have resulted in development of resistance among the microbes that could transfer resistance factors to another bacteria, making consumers vulnerable to the resistant bacteria. Therefore this study investigated the resistance profile of Escherichia coli isolated from 4 Catfish ponds water in Ago-Iwoye, Nigeria. The E. coli were identified morphologically and Biochemically. The total microbial load of the fish ponds water sampled ranged from 6.1 × 103 Cfu/ml to 9.1×103 Cfu/ml. E. coli strains were highly resistant to Cloxacillin (100%), Streptomycin (100%), Tetracycline (100%), Chloramphenicol (100%) and Augumentin (90%). The isolates were sensitive to Ofloxacin (35%), Ceftazidine (40%) and Gentamicin (28%). While the study concluded that catfish ponds water are unhygienic and can cause harm to fish reared in the water and those that consume catfish, long-age practice of using antibiotics as growth promoter in agriculture should be discouraged.
Keywords: Escherichia coli, Antibiotic resistance, Catfish Pond
TABLE OF CONTENT
CHAPTER ONE: INTRODUCTION
1.1 Background to the Study
1.2 Objectives of the Study
1.3 Justification
1.4 Literature Review
CHAPTER TWO: MATERIALS AND METHODS
2.1 Selection of Sampling Area
2.2 Collection of Samples
2.3 Material and Apparatus Used
2.4 Sterilization of Glasswares
2.5 Media Used
2.6 Media Preparation
2.7 Determination of Microbial Load
2.8 Isolation of Escherichia coli
2.9 Identification and Characterization of Escherichia coli
2.9.1 Catalase Test
2.9.2 Indole Test
2.9.3 Methyl Red Test
3.9.4 Voges Proskauer Test
2.9.5 Citrate Utilisation
2.10 Antibiotic Susceptibility Test
2.12 Determination of Multiple Antibiotic Resistances
CHAPTER THREE: RESULTS
CHAPTER FOUR: DISCUSSION, CONCLUSION AND RECOMMENDATION
4.1 Discussion
4.2 Conclusion
4.3 Recommendations
REFERENCES
LIST OF TABLES
3.1 Total Microbial Load of Organisms on MacConkey Agar
3.2 Biochemical characterization of Escherichia coli isolated from Cat Fish Pond Water
3.3 Antibiotics Resistance of Escherichia coli on Selected Antibiotics
LIST OF FIGURES
3.1 Percentage of Escherichia coli resistance to antibiotics
3.2 Growth of E. coli on EMB
3.3 Showing the susceptibility and resistance of E. coli to different antibiotics
3.4 Showing the susceptibility and resistance of E. coli to different antibiotics.
