A STUDY OF GENETICAL DIVERSITY OF HONEY BEE

A STUDY OF GENETICAL DIVERSITY OF HONEY BEE

Project Details

A STUDY OF GENETICAL DIVERSITY OF HONEY BEE


45 Pages, Chapter 1-5
Ms Word

08062235854

Contact Chris Research

Description

A study of genetic diversity of African honey bee (Apis mellifera adansonii) was carried out on twenty two (22) bee colonies from four (4) different bee farms in Ogun environ. The extracted DNA was pure and of good quality using modified Murray and Thompson technique (1980). The worker bee samples were amplified using seven (7) primers (A81, A29, A113, A107, A107, A14, IC1 and B124). A total of 81 bands amplified for all primers except B124 that showed no bands. 80 bands were polymorphic, while 1 was monomorphic, the polymorphic bands ranged between 10 and 16 per primer. The bands were scored for their presence (1) and absence (0), the score was then used in constructing a dendogram. Factorial analysis further confirmed the genetic variability of all the honeybee samples from the 22 colonies. The genetic composition of the worker bees of Apis mellifera adansonii in Ijebu  environ using sample sequence repeat (SSR) revealed that no two (2) sample of the population has the same genetic composition, yet they all belong to a genetic lineage.

TABLE OF CONTENT

CHAPTER ONE

  • Introduction

1.1       Classification of honeybee

1.2       Stages of development

CHAPTER TWO:

  • Literature review

2.1       Genetic diversity of Apis mellifera                                                                

2.2       Morphological analysis of Apis mellifera                                                       

2.3       Molecular analysis of Apis mellifera                                                              

2.3.1    PCR based method

2.3.2    Non-PCR based methods

CHAPTER THREE:                                                                                                           

  • Materials

3.1       Methods

3.1.1    Collection of samples

3.1.2    Sampling and DNA extraction

3.1.3    Dilution of primers

3.1.4    Amplification and gel electrophoresis

3.1.5    Data analysis

CHAPTER FOUR:

  • Results

4.1       Plates and Tables

4.2       Dendogram

4.3       Factorial analysis

CHAPTER FIVE

  • Discussion

5.1       Conclusion

References                                                                                                                

Appendix                                                                                                                  

LIST OF TABLES

Table 1:           Roles of bees depending on gender

Table 2:           Classical morphometrics and routine

Table 3:           Preparation of buffer

Table 4:           Dilution of primer

Table 5:           Scoring sheet showing amplification of primer A81

Table 6:           Scoring sheet showing amplification of primer A29

Table 7:           Scoring sheet showing amplification of primer IC1

Tables 8:          Scoring sheet showing amplification of primer A113

Table 9:           Scoring sheet showing amplification of primer A107

Table 10:         Scoring sheet showing amplification of primer A14

LIST OF FIGURES

Figure 1:          Development of markers

Figure 2:          Queen bee

Figure 3:          Worker bee

Figure 4:          Drone bee

Figure 5:          Dendogram showing the relationship of similarities and

differences among the 22 samples of worker bees

Figure 6:          Factorial analysis showing the genetic diversity of 44 worker

bee samples based on their genetic diversity.

Figure 7:          Factorial analysis showing the genetic diversity of 44 worker

bee samples based on their genetic diversity and locations.

Figure 8:          Statistical analysis

LIST OF PLATES

Plate 1:            Amplification of 44 worker bee samples using primer B124

Plate 2:            Amplification of 44 worker bee samples using primer A81

Plate 3:            Amplification of 44 worker bee samples using primer A29

Plate 4:            Amplification of 44 worker bee samples using primer IC1

Plate 5:            Amplification of 44 worker bee samples using primer A113

Plate 6:            Amplification of 44 worker bee samples using primer A107

Plate 7:            Amplification of 44 worker bee samples using primer A14

  •  
  •  
  •  
  •  
  •  
  •  

Amenities

Submit Your Review

Leave a Reply

Your email address will not be published. Required fields are marked *

Claim Listing